---
title: Kovac’s reagent
description: "Kovac’s reagent consists of p-dimethylaminobenzaldehyde dissolved in HCl. Five drops or so of the reagent are added to an overnight culture grown in [tryptone broth](https://vumicro.com/docs/tryptone-broth/). Development of a red ring atop the broth is indicative of the production of [indole](https://vumicro.com/docs/indole-production-test/),…"
url: "https://vumicro.com/es/docs/kovacs-reagent/"
updated: "2022-06-17"
category: Reference Library / Reagents, Chemicals, Stains
---

# Kovac’s reagent

Kovac’s reagent consists of p-dimethylaminobenzaldehyde dissolved in HCl.

Five drops or so of the reagent are added to an overnight culture grown in [tryptone broth](https://vumicro.com/docs/tryptone-broth/). Development of a red ring atop the broth is indicative of the production of [indole](https://vumicro.com/docs/indole-production-test/), a breakdown product in the digestion of the amino acid tryptophan.

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