---
title: Nitrate B reagent
description: "Nitrate B reagent consists of sulfanilic acid dissolved in acetic acid. It is used with Nitrate A reagent (dimethyl-naphthylamine or alpha-naphthylamine dissolved in acetic acid) to detect the presence of nitrite produced by the [reduction of nitrate](https://vumicro.com/docs/nitrate-reductase-test/) in [nitrate broth](https://vumicro.com/docs/nitrate-broth-with-durham-tube/).…"
url: "https://vumicro.com/es/docs/nitrate-b-reagent/"
updated: "2022-06-17"
category: Reference Library / Reagents, Chemicals, Stains
---

# Nitrate B reagent

Nitrate B reagent consists of sulfanilic acid dissolved in acetic acid. It is used with Nitrate A reagent (dimethyl-naphthylamine or alpha-naphthylamine dissolved in acetic acid) to detect the presence of nitrite produced by the [reduction of nitrate](https://vumicro.com/docs/nitrate-reductase-test/) in [nitrate broth](https://vumicro.com/docs/nitrate-broth-with-durham-tube/).

After inoculating nitrate broth and incubating overnight, five drops of each reagent are added to the culture. Development of a red color indicates the nitrate was reduced to nitrite.

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