This test determines whether the microbe can grow in a medium where potassium cyanide is present as a carbon and nitrogen source.<\/p>\n\n\n\n
If the microbe can grow in the presence of potassium cyanide , the broth will become turbid (cloudy) after incubation.<\/p>\n\n\n\n
The medium used is Potassium Cyanide (KCN) broth<\/a> , a nutrient medium with 0.75% potassium cyanide added.<\/p>\n\n\n\n An inoculum from a pure culture is transferred aseptically to a sterile tube of KCN broth<\/a>. The inoculated tube is incubated at 35-37 C for 24 hours. A positive test is indicated by the presence of turbidity.<\/p>\n\n\n\n None.<\/p>\n\n\n\n 1. Select the potassium cyanide (KCN) broth<\/a> medium. <\/p>\n\n\n\n 2. Complete the process of a tube-to-tube aseptic transfer<\/a> to inoculate the medium.<\/p>\n\n\n\n 3. Place the inoculated tube into the 35-37 C incubator. <\/p>\n\n\n\n 4. Press the New Day button<\/a> to move forward 24 hours.<\/p>\n\n\n\n 5. Incubate this test for only 24 hours. <\/p>\n\n\n\n 6. Retrieve the incubated culture from the incubator.<\/p>\n\n\n\n 7. Observe test result. The test is positive if there is obvious turbidity in the tube. In negative tests, there is no growth. <\/p>\n\n\n\n 8.\u00a0Record test result<\/a>. \u00a0<\/p>\n\n\n\n Uninoculated KCN broth<\/a><\/p>\n\n\n\nHow is the test performed? <\/h3>\n\n\n\n
What reagents are added? <\/h3>\n\n\n\n
Performing this test in the VUMIE Online lab<\/h2>\n\n\n\n
Inoculation of Medium<\/h3>\n\n\n\n
Incubation of the Inoculated Medium<\/h3>\n\n\n\n
Determination of Test Results<\/h3>\n\n\n\n