The purpose is to see if the microbe can use corn oil, a lipid, as a source of carbon and energy for growth. Use of corn oil is accomplished by a class of enzymes called lipases .<\/p>\n\n\n\n
A medium containing lipids (in this case corn oil ) and an indicator is used. When lipids are digested, the indicator changes from blue to clear. Halos surrounding colonies is indicative of their ability to digest the lipid in the medium due to the presence of lipase .<\/p>\n\n\n\n
The medium used is spirit blue agar plate<\/a>. The medium is a nutrient agar to which emulsified corn oil is added after the medium has been sterilized. The indicator spirit blue produces a deep blue medium that changes to a light blue opaque medium after addition of the lipid .<\/p>\n\n\n\n An inoculum from a pure culture is streaked on a sterile plate of spirit blue agar<\/a>. The inoculated plate is incubated at 35-37 C for 24 hours. Presence of halos surrounding colonies is positive for their ability to digest the lipids and thus indicates presence of lipase.<\/p>\n\n\n\n None.<\/p>\n\n\n\n 1. Select the spirit blue agar plate<\/a> medium. <\/p>\n\n\n\n 2. Using an inoculating loop, complete the process of a tube-to-plate aseptic transfer<\/a> to inoculate the medium. <\/p>\n\n\n\n 3. Place the inoculated tube into the 35-37 C incubator. <\/p>\n\n\n\n 4. Press the New Day button<\/a> to move forward 24 hours.<\/p>\n\n\n\n 5. Incubate this test for only 24 hours. <\/p>\n\n\n\n 6. Retrieve the incubated culture from the incubator.<\/p>\n\n\n\n 14. Incubate for the appropriate length of time. For this test, 24 hours is sufficient for determining the result. <\/p>\n\n\n\n 15. Retrieve desired incubated culture from the incubator. <\/p>\n\n\n\n 16. Observe the test result, noting specifically the color of the medium surrounding colonies in the plate. The culture will show clear halos around colonies capable of breaking down lipids. The color surrounding colonies without lipase remains unchanged. <\/p>\n\n\n\n 17. Record test result<\/a>. <\/p>\n\n\n\n Uninoculated Spirit Blue agar plate:<\/a><\/p>\n\n\n\nHow is the test performed? <\/h3>\n\n\n\n
What reagents are added? <\/h3>\n\n\n\n
Performing this test in the VUMIE Online lab<\/h2>\n\n\n\n
Inoculation of Medium<\/h3>\n\n\n\n
Incubation of the Inoculated Medium<\/h3>\n\n\n\n
Determination of Test Results<\/h3>\n\n\n\n
Determination of Test Results<\/h3>\n\n\n\n