Oxidase Test

 

 

In VUMIE Online© Online Lab a video is provided that depicts removal of cells from an isolated colony of the unknown microbe and placement on a piece of filter paper for performing the test.  Oxidase reagent  is then added and the reaction is observed.  

 

To view the oxidase test in VUMIE Online© Online Lab , complete the following steps:

1. Open the Tests link above the Virtual lab and select the Oxidase Video.  

2. Press the Play button to watch the video. A short video clip showing microscopic observation of an oxidase  test for the unknown organism will play.

3. Observe the results. If the bacterium possesses oxidase , the smudge of bacteria will turn the oxidase reagent on the filter paper indigo blue in a few seconds.  If this does not occur, the microbe is oxidase negative.

4. Record the test result observed .