Indole Production Test

 

 

To perform this test in VUMIE Online© Online Lab , complete the following steps:

 

Inoculation of Medium

1. Select the tryptone broth medium.  

2. Complete the process of a tube-to-tube aseptic transfer to inoculate the medium.  Forgotten how to do these things?  Watch the "Show Me How To" videos.

 

Incubation of the Inoculated Medium

3. Place the inoculated tube into the 35-37 C incubator.  

  4. Press the New Day button to move forward 24 hours.  Forgotten how to do these things?  Watch the "Show Me How To" videos.

 

Addition of Reagents

5. Incubate this test for only 24 hours.  

6.  Retrieve the incubated culture from the incubator.

7.  This is a test that requires addition of reagents to bring about the change in appearance of a positive test. 

 

 

Select the dropper tool and the appropriate reagent needed from the chemical shelf. For this test, select Kovac’s.  

 

 

  8. Remove the cap from the tube.  

  9. Place the end of the dropper into the tube and add the reagent to the culture.  

 

Determination of Test Results

10. Observe test result. For this test, addition of the appropriate reagent produces a cherry-red ring floating above the culture medium if the test is positive. If negative, the ring that forms is yellow or amber.  

11. Record test result.