Lactose Fermentation Test

 

 

To perform this test in VUMIE Online© Online Lab , complete the following steps:

 

Inoculation of Medium

1. Select the phenol red lactose broth medium.  

2. Complete the process of a tube-to-tube aseptic transfer to inoculate the medium.  Forgotten how to do these things?  Watch the "Show Me How To" videos.

 

Incubation of the Inoculated Medium

3. Place the inoculated tube into the 35-37 C incubator.  

  4. Press the New Day button to move forward 24 hours.  Forgotten how to do these things?  Watch the "Show Me How To" videos.

 

Determination of Test Results

5. Incubate this test for only 24 hours.  

6.  Retrieve the incubated culture from the incubator.

7. No reagents are required for completion of this test. 

8. Positive tests will be yellow; negative results are any other color.

  9.  Record test result.  Forgotten how to do these things?  Watch the "Show Me How To" videos.