OF Glucose Test

 

 

To perform the OF Glucose – Fermentation test in VUMIE Online© Online Lab , complete the following steps:

 

Inoculation of Medium

1. Select the OF glucose with oil overlay medium.  

2. Complete the process of a tube-to-tube aseptic transfer to inoculate the medium.  Forgotten how to do these things?  Watch the "Show Me How To" videos.

 

Incubation of the Inoculated Medium

3. Place the inoculated tube into the 35-37 C incubator.  

  4. Press the New Day button to move forward 24 hours.  Forgotten how to do these things?  Watch the "Show Me How To" videos.

 

Determination of Test Results

5. Incubate this test for only 24 hours.  

6.  Retrieve the incubated culture from the incubator.

 

Determination of Test Results

14. Incubate for the appropriate length of time. For this test, 24 hours is sufficient for determining the result.  

15. Retrieve desired incubated culture from the incubator.  

16. Observe test result. If the test was followed as described above, the culture will have changed to yellow in the presence of acids (indicating a positive test) or remain green in the presence of bases/alkali (indicating a negative test).  

17. Record test result.  

18. Dispose of the culture.