Biochemical Tests

"Show me how to" videos

29

Add reagents
Change a test result
Choose a test/medium
Create a new unknown
Declare the ID of a microbe
Dispose of cultures
Find a previously created unknown
Flame lab tools
Flame tube mouths
Identify gram negative enteric bacilli
Identify gram positive cocci
Incubate cultures
Perform a gram stain
Perform aseptic transfers (create lawns)
Perform aseptic transfers (tube-to-plate)
Perform aseptic transfers (tube-to-tube)
Perform video-based tests
Record test results
Remove and replace caps and lids
Repeat a test
Select a lab tool
Start over with a new unknown
Start the incinerator
Use a strategy to identify bacteria
Use auto-inoculation
Use the “traffic signals”
Use the identification matrix
Use the New Day button
View the virtual lab report

Admin tutorial

7

1) Logging in as an instructor
2) Creating a section
3) Enrolling students in a section
4) Adding activities
5) Viewing student grades
6) Editing gradebook preferences
7) Canvas integration

Antibiotics

12

Bacitracin
Chloramphenicol
Erythromycin
Kanamycin
Nalidixic Acid
Nitrofurantoin
Novobiocin
Optochin
Penicillin
Streptomycin
Sulfamethoxazole
Tetracycline

63

Acid from glucose
Adonitol fermentation test
Antibiotic susceptibility tests
Arabinose fermentation test
Arabitol fermentation test
Arginine dihydrolase test
Bacitracin susceptibility test
Catalase test
Cellobiose fermentation test
Citrate utilization test
Coagulase test
Deoxyribonuclease (DNase) test
Dulcitol fermentation test
Esculin hydrolysis test
Fructose fermentation test
Galactose fermentation test
Gas from glucose
Gelatin hydrolysis test
Glucose fermentation
Glycerol fermentation test
Growth in KCN (potassium cyanide)
Growth on 6.5% NaCl
Growth on 7.5% NaCl
Growth on bile salts test
Hand Hygiene and Infection Control
Hemolysis on blood agar
Hippurate Hydrolysis Test
Hydrogen sulfide (H2S) production test
Indole production test
Inositol fermentation
Kirby-Bauer Antibiotic Susceptibility Test
Lactose fermentation test
Lipase test (using corn oil)
Lysine decarboxylase test
Malonate utilization test
Maltose fermentation test
Mannitol fermentation test
Mannose fermentation test
Melezitose fermentation test
Melibiose fermentation test
Methyl red test
Motility test
myo-Inositol fermentation test
Nitrate reductase test
Novobiocin susceptibility test
OF glucose test
Optochin susceptibility test
Ornithine decarboxylase test
Oxidase test
Oxygen Requirements Determination
Phenylalanine deaminase test
Raffinose fermentation test
Rhamnose fermentation test
Ribose fermentation test
Salicin fermentation test
Sorbitol fermentation test
Starch hydrolysis test
Sucrose fermentation test
Trehalose fermentation test
Urea hydrolysis test (Christiansen’s)
Voges-Proskauer test
Xylitol fermentation test
Xylose fermentation test

Gram negative bacilli

76

Buttiauxella agrestis
Cedecea davisae
Cedecea lapagei
Cedecea neteri
Citrobacter amalonaticus
Citrobacter amalonaticus biogroup 1
Citrobacter freundii
Citrobacter koseri
Cronobacter sakazakii
Edwardsiella hoshinae
Edwardsiella ictaluri
Edwardsiella tarda (wild type)
Edwardsiella tarda biogroup 1
Enterobacter aerogenes
Enterobacter amnigenus biogroup 1
Enterobacter amnigenus biogroup 2
Enterobacter cancerogenus
Enterobacter cloacae
Enterobacter gergoviae
Escherichia coli
Escherichia fergusonii
Escherichia hermannii
Escherichia vulneris
Ewingella americana
Hafnia alvei
Klebsiella oxytoca
Klebsiella pneumoniae subspecies ozaenae
Klebsiella pneumoniae subspecies pneumoniae
Klebsiella pneumoniae subspecies rhinoscleromatis
Kluyvera ascorbata
Kluyvera intermedia
Leclercia adecarboxylata
Mollerella wisconsinsis
Morganella morganii subspecies morganii
Morganella morganii subspecies sibonii
Proteus mirabilis
Proteus myxofaciens
Proteus penneri
Proteus vulgaris
Providencia alcalifaciens
Providencia rettgeri
Providencia rustigianii
Providencia stuartii
Rahnella aquatilis
Raoultella ornithinolytica
Raoultella terrigena
Salmonella bongori
Salmonella enterica
Salmonella enterica subspecies arizonae
Salmonella enterica subspecies enterica
Salmonella enterica subspecies houtenae
Salmonella enterica subspecies paratyphi
Salmonella enterica subspecies salamae
Salmonella enteritidis
Salmonella montevideo
Salmonella newport
Salmonella typhi
Salmonella typhimurium
Serratia ficaria
Serratia fonticola
Serratia marcescens
Serratia odorifera biogroup 1
Serratia plymuthica
Serratia proteamaculans subspecies proteamaculans
Serratia rubidaea
Shigella boydii
Shigella dysenteriae
Shigella flexneri
Shigella sonnei
Shimwellia blattae
Tatumella ptyseos
Yersinia enterocolitica
Yersinia frederiksenii
Yersinia kristensenii
Yersinia pestis
Yersinia pseudotuberculosis

Gram positive bacilli

10

Bacillus anthracis
Bacillus cereus
Bacillus licheniformis
Bacillus megaterium
Bacillus subtilis
Bacillus thuringiensis
Listeria innocua
Listeria ivanovii
Listeria monocytogenes
Listeria seeligeri

Gram positive cocci

53

Dermacoccus nishinomiyaensis
Enterococcus avium
Enterococcus faecalis
Enterococcus faecium
Enterococcus gallinarum
Kocuria kristinae
Kocuria rosea
Kocuria varians
Kytococcus sedentarius
Lactococcus lactis
Lactococcus raffinolactis
Macrococcus caseolyticus
Micrococcus luteus
Micrococcus lylae
Nesterenkonia halobia
Rothia mucilaginosa
Staphylococcus aureus
Staphylococcus auricularis
Staphylococcus capitis
Staphylococcus caprae
Staphylococcus carnosus
Staphylococcus chromogenes
Staphylococcus cohnii ssp. cohnii
Staphylococcus cohnii ssp. urealyticum
Staphylococcus epidermidis
Staphylococcus gallinarum
Staphylococcus haemolyticus
Staphylococcus hominis
Staphylococcus hyicus
Staphylococcus intermedius
Staphylococcus lentus
Staphylococcus saccharolyticus
Staphylococcus saprophyticus
Staphylococcus sciuri
Staphylococcus simulans
Staphylococcus warneri
Staphylococcus xylosus
Streptococcus acidominimus
Streptococcus agalactiae
Streptococcus bovis
Streptococcus criceti
Streptococcus equi
Streptococcus equinus
Streptococcus ferus
Streptococcus iniae
Streptococcus mutans
Streptococcus pneumoniae
Streptococcus pyogenes
Streptococcus ratti
Streptococcus salivarius
Streptococcus sanguinis
Streptococcus sobrinus
Streptococcus uberis

Media

52

6.5% NaCl broth
Arginine dihydrolase broth
Bile esculin agar slant
Blood agar
Christiansen’s urea broth
DNase agar with methyl green plate
Fluid Thioglycolate
Lysine decarboxylase broth
Malonate broth
Mannitol salt agar
MRVP broth
Mueller Hinton Agar
Nitrate broth with Durham tube
Nutrient agar plate
Nutrient broth
Nutrient gelatin
OF glucose broth
Ornithine decarboxylase broth
Phenol red adonitol broth
Phenol red arabinose broth
Phenol red arabitol broth
Phenol red cellobiose broth
Phenol red dulcitol broth
Phenol red fructose (levulose) broth
Phenol red galactose broth
Phenol red glucose (dextrose) broth with Durham tube
Phenol red glycerol broth
Phenol red inositol broth
Phenol red lactose broth
Phenol red maltose broth
Phenol red mannitol broth
Phenol red mannose broth
Phenol red melezitose broth
Phenol red melibiose broth
Phenol red raffinose broth
Phenol red rhamnose broth
Phenol red ribose broth
Phenol red salicin broth
Phenol red sorbitol broth
Phenol red sucrose (saccharose) broth
Phenol red trehalose broth
Phenol red xylitol broth
Phenol red xylose broth
Phenylalanine agar slant
Potassium cyanide (KCN) broth
Rabbit plasma
Simmons’ citrate agar slant
Sodium Hippurate Medium
Spirit blue agar plate
Starch agar
Triple sugar iron agar slant
Tryptone broth

Quick start tutorial

4

Creating a user account
Lab layout
Navigating VUMIE’s main features
Sections, Activities, and Quizzes

Reagents, Chemicals, Stains

19

Ammonium Sulfate (saturated)
Barritt’s A reagent
Barritt’s B reagent
Crystal Violet
Ferric chloride (10%) reagent
Gram’s Decolorizer
Gram’s Iodine
HCl 0.1N reagent
Hydrogen peroxide
Iodine reagent
Kovac’s reagent
Malachite Green
Methyl red reagent
Ninhydrin
Nitrate A reagent
Nitrate B reagent
Oxidase reagent
Rabbit Plasma
Safranine

Spiral and Curved Bacteria

10

Campylobacter coli
Campylobacter fetus
Campylobacter jejuni
Campylobacter lari
Campylobacter mucosalis
Vibrio cholerae
Vibrio fluvialis
Vibrio mimicus
Vibrio parahaemolyticus
Vibrio vulnificus

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Biochemical Tests

Catalase test