VUMIE Online

Gram Stains
Microscopy
Anatomy, Shape, Arrangements

Objective quiz – Types of media

Section 1

Indicate which of the following statements about media are true.

1. Broth media are agars dispensed into test tubes rather than plates
2. Media in Petri dishes are the only media dispensed into a container after autoclaving
3. Agar is a seaweed extract that provides nutrients in plated media
4. The standard amount of agar added to make a broth semisolid is 15 g per 100 ml
5. When putting media into culture tubes, both tubes and media are sterilized together
6. Growth media are often over 90% water
7. A sterile blade is used to cut agar in a tube to make an agar slant
8. Inhibitors make a medium differential
9. Enrichments are added to support fastidious organisms
10. Differential media typically change colors only when a specific chemical reaction takes place

Section 2

Put the steps in order for each question below.

11. Put the following steps in preparation for broth media in their correct order.
  • Allow to cool
  • Use for culturing cells
  • Mix ingredients
  • Autoclave
  • Dispense into tubes
12. Put the following steps in preparation for agar slants in their correct order.
  • Use for culturing cells
  • Autoclave
  • Dispense into tubes
  • Mix ingredients
  • Allow to solidify
13. Put the following steps in preparation of plated agar in their correct order.
  • Use for culturing cells
  • Dispense into plates
  • Autoclave
  • Allow to solidify
  • Mix ingredients

Uncategorized

14. Which of the following is not a standard type of growth medium?