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Gram Stains
Microscopy
Anatomy, Shape, Arrangements

Worksheet – Types of media

Section 1

Indicate where each protocol described below would be used for preparation of these media:
A. Making agar media for plates
B. Making broth media for tubes
C. Making agar media for tubes
D. Not used for any of these

1. Broth in flask, add agar, heat to dissolve, autoclave, dispense into containers

2. Broth in flask, heat to dissolve, autoclave, add agar, dispense into containers

3. Broth in flask, heat to dissolve, dispense into containers, autoclave, add agar

4. Broth in flask, add agar, heat to dissolve, dispense into containers, autoclave

5. Broth in flask, heat to dissolve, autoclave, dispense into containers

6. Broth in flask, heat to dissolve, dispense into containers, autoclave

7. Broth in flask, heat to dissolve, add agar, autoclave, dispense into containers

8. Broth in flask, dispense into containers, heat to dissolve, add agar, autoclave

Section 2

Indicate which of the following statements about media are true.

9. Broth media can be agars dispensed into test tubes rather than plates

10. Tubes may contain broth or agar media, but Petri plates only contain agars

11. Media in Petri dishes are the only media dispensed into a container after autoclaving

12. Agar is a seaweed extract that provides no nutrients in plated media

13. The standard amount of agar added to make a broth semisolid is 15 g per 100 ml

14. When putting media into culture tubes, both tubes and media are sterilized together

15. When putting media into Petri plates, the media are in the Petri dishes during sterilization

16. Agar slants are made by tilting the tubes in which the agar is allowed to solidify

17. Inhibitors make a medium differential

18. Indicators are added to support the growth of fastidious organisms

19. Differential media typically change colors only when a specific chemical reaction takes place

Uncategorized

20. How much agar would be added to 100 ml of broth to convert it to a semi-solid medium?

21. How much agar would be added to 500 ml of broth to convert it to a semi-solid medium?