Objective Quiz – Tube-Tube Transfers

Indicate all correct answers.

1. Tube-to-Tube transfers are used:

For isolating bacteria

For purifying bacteria

For studying bacteria

For maintaining viable cultures of bacteria

For testing to identifying bacteria

2. A problem in using a mixed culture for identifying an unknown is:

You don’t know which microbe was responsible for a positive test result

You don’t know which microbe was responsible for a negative test result

You will have inconsistent results in repeated tests

A biochemical test medium can only support the growth of one species at a time

Pathogens will not grow in mixed cultures

3. A goal of aseptic technique is to:

Not contaminate the culture of unknown being studied

Not contaminate the environment where the culture is being studied

Keep the inoculum of your unknown sterile

Put only bacteria from your inoculum into the sterile broth

Use one pure culture to create another pure culture

4. The first and last steps of every tube-to-tube transfer are:

Removing and replacing caps on tubes

Flaming the mouths of tubes

Flaming the loop

Putting the tube in the incubator

Reading the results of the test

5. Indicate which of the following statements are True:

Always flame your loop in between picking up inoculum and depositing it in the sterile tube

Always flame your tubes in between picking up inoculum and depositing it in the sterile tube

Always flame mouths of tubes immediately after removing caps and before replacing them

Always place the tube caps on the lab bench during an aseptic transfer

Always incubate cultures overnight before evaluating the results of a test

Always hold both inoculum tube and sterile medium throughout the transfer process

Always replace the inoculum cap before removing the cap from the sterile tube

Always turn off the incinerator before leaving the VUMIE Online lab overnight

6. Place these steps in an aseptic tube-to-tube transfer in their proper order of occurrence: