Barritt’s B reagent consists of 40% potassium hydroxide (KOH). It is used with Barritt’s A reagent (alpha-naphthol dissolved in ethanol) to detect the presence of 2,3-butanediol (so-called “neutral fermentation products”) produced from glucose in MRVP broth.
After inoculating MRVP broth and incubating overnight, five drops of each reagent are added to the culture. Barritt’s A should be added first. This is followed by addition of five drips of Barritt’s B. Development of a red color indicates the presence of 2,3-butanediol.
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