Antibiotics

  • Bacitracin
  • Chloramphenicol
  • Erythromycin
  • Kanamycin
  • Nalidixic Acid
  • Nitrofurantoin
  • Novobiocin
  • Optochin
  • Penicillin
  • Streptomycin
  • Sulfamethoxazole
  • Tetracycline

Biochemical tests

  • Acid from glucose
  • Adonitol fermentation test
  • Antibiotic susceptibility tests
  • Arabinose fermentation test
  • Arabitol fermentation test
  • Arginine dihydrolase test
  • Bacitracin susceptibility test
  • Catalase test
  • Cellobiose fermentation test
  • Citrate utilization test
  • Coagulase test
  • Deoxyribonuclease (DNase) test
  • Dulcitol fermentation test
  • Esculin hydrolysis test
  • Fructose fermentation test
  • Galactose fermentation test
  • Gas from glucose
  • Gelatin hydrolysis test
  • Glucose fermentation
  • Glycerol fermentation test
  • Growth in KCN (potassium cyanide)
  • Growth on 6.5% NaCl
  • Growth on 7.5% NaCl
  • Growth on bile salts test
  • Hand Hygiene and Infection Control
  • Hemolysis on blood agar
  • Hydrogen sulfide (H2S) production test
  • Indole production test
  • Inositol fermentation
  • Kirby-Bauer Antibiotic Susceptibility Test
  • Lactose fermentation test
  • Lipase test (using corn oil)
  • Lysine decarboxylase test
  • Malonate utilization test
  • Maltose fermentation test
  • Mannitol fermentation test
  • Mannose fermentation test
  • Melezitose fermentation test
  • Melibiose fermentation test
  • Methyl red test
  • Motility test
  • myo-Inositol fermentation test
  • Nitrate reductase test
  • Novobiocin susceptibility test
  • OF glucose test
  • Optochin susceptibility test
  • Ornithine decarboxylase test
  • Oxidase test
  • Phenylalanine deaminase test
  • Raffinose fermentation test
  • Rhamnose fermentation test
  • Ribose fermentation test
  • Salicin fermentation test
  • Sorbitol fermentation test
  • Starch hydrolysis test
  • Sucrose fermentation test
  • Trehalose fermentation test
  • Urea hydrolysis test (Christiansen’s)
  • Voges-Proskauer test
  • Xylitol fermentation test
  • Xylose fermentation test

Gram negative bacilli

  • Buttiauxella agrestis
  • Cedecea davisae
  • Cedecea lapagei
  • Cedecea neteri
  • Citrobacter amalonaticus
  • Citrobacter amalonaticus biogroup 1
  • Citrobacter freundii
  • Citrobacter koseri
  • Cronobacter sakazakii
  • Edwardsiella hoshinae
  • Edwardsiella ictaluri
  • Edwardsiella tarda (wild type)
  • Edwardsiella tarda biogroup 1
  • Enterobacter aerogenes
  • Enterobacter amnigenus biogroup 1
  • Enterobacter amnigenus biogroup 2
  • Enterobacter cancerogenus
  • Enterobacter cloacae
  • Enterobacter gergoviae
  • Escherichia coli
  • Escherichia fergusonii
  • Escherichia hermannii
  • Escherichia vulneris
  • Ewingella americana
  • Hafnia alvei
  • Klebsiella oxytoca
  • Klebsiella pneumoniae subspecies ozaenae
  • Klebsiella pneumoniae subspecies pneumoniae
  • Klebsiella pneumoniae subspecies rhinoscleromatis
  • Kluyvera ascorbata
  • Kluyvera intermedia
  • Leclercia adecarboxylata
  • Mollerella wisconsinsis
  • Morganella morganii subspecies morganii
  • Morganella morganii subspecies sibonii
  • Proteus mirabilis
  • Proteus myxofaciens
  • Proteus penneri
  • Proteus vulgaris
  • Providencia alcalifaciens
  • Providencia rettgeri
  • Providencia rustigianii
  • Providencia stuartii
  • Rahnella aquatilis
  • Raoultella ornithinolytica
  • Raoultella terrigena
  • Salmonella bongori
  • Salmonella enterica
  • Salmonella enterica subspecies arizonae
  • Salmonella enterica subspecies enterica
  • Salmonella enterica subspecies houtenae
  • Salmonella enterica subspecies paratyphi
  • Salmonella enterica subspecies salamae
  • Salmonella typhi
  • Serratia ficaria
  • Serratia fonticola
  • Serratia marcescens
  • Serratia odorifera biogroup 1
  • Serratia plymuthica
  • Serratia proteamaculans subspecies proteamaculans
  • Serratia rubidaea
  • Shigella dysenteriae
  • Shigella flexneri
  • Shigella sonnei
  • Shimwellia blattae
  • Tatumella ptyseos
  • Yersinia enterocolitica
  • Yersinia frederiksenii
  • Yersinia kristensenii
  • Yersinia pestis
  • Yersinia pseudotuberculosis

Gram positive cocci

  • Dermacoccus nishinomiyaensis
  • Enterococcus avium
  • Enterococcus faecalis
  • Enterococcus faecium
  • Enterococcus gallinarum
  • Kocuria kristinae
  • Kocuria rosea
  • Kocuria varians
  • Kytococcus sedentarius
  • Lactococcus lactis
  • Lactococcus raffinolactis
  • Macrococcus caseolyticus
  • Micrococcus luteus
  • Micrococcus lylae
  • Nesterenkonia halobia
  • Rothia mucilaginosa
  • Staphylococcus aureus
  • Staphylococcus auricularis
  • Staphylococcus capitis
  • Staphylococcus caprae
  • Staphylococcus carnosus
  • Staphylococcus chromogenes
  • Staphylococcus cohnii ssp. cohnii
  • Staphylococcus cohnii ssp. urealyticum
  • Staphylococcus epidermidis
  • Staphylococcus gallinarum
  • Staphylococcus haemolyticus
  • Staphylococcus hominis
  • Staphylococcus hyicus
  • Staphylococcus intermedius
  • Staphylococcus lentus
  • Staphylococcus saccharolyticus
  • Staphylococcus saprophyticus
  • Staphylococcus sciuri
  • Staphylococcus simulans
  • Staphylococcus warneri
  • Staphylococcus xylosus
  • Streptococcus acidominimus
  • Streptococcus agalactiae
  • Streptococcus bovis
  • Streptococcus criceti
  • Streptococcus equi
  • Streptococcus equinus
  • Streptococcus ferus
  • Streptococcus iniae
  • Streptococcus mutans
  • Streptococcus pneumoniae
  • Streptococcus pyogenes
  • Streptococcus ratti
  • Streptococcus salivarius
  • Streptococcus sanguinis
  • Streptococcus sobrinus
  • Streptococcus uberis

Media

  • 6.5% NaCl broth
  • Arginine dihydrolase broth
  • Bile esculin agar slant
  • Blood agar
  • Christiansen’s urea broth
  • DNase agar with methyl green plate
  • Lysine decarboxylase broth
  • Malonate broth
  • Mannitol salt agar
  • MRVP broth
  • Mueller Hinton Agar
  • Nitrate broth with Durham tube
  • Nutrient agar plate
  • Nutrient broth
  • Nutrient gelatin
  • OF glucose broth
  • Ornithine decarboxylase broth
  • Phenol red adonitol broth
  • Phenol red arabinose broth
  • Phenol red arabitol broth
  • Phenol red cellobiose broth
  • Phenol red dulcitol broth
  • Phenol red fructose (levulose) broth
  • Phenol red galactose broth
  • Phenol red glucose (dextrose) broth with Durham tube
  • Phenol red glycerol broth
  • Phenol red inositol broth
  • Phenol red lactose broth
  • Phenol red maltose broth
  • Phenol red mannitol broth
  • Phenol red mannose broth
  • Phenol red melezitose broth
  • Phenol red melibiose broth
  • Phenol red raffinose broth
  • Phenol red rhamnose broth
  • Phenol red ribose broth
  • Phenol red salicin broth
  • Phenol red sorbitol broth
  • Phenol red sucrose (saccharose) broth
  • Phenol red trehalose broth
  • Phenol red xylitol broth
  • Phenol red xylose broth
  • Phenylalanine agar slant
  • Potassium cyanide (KCN) broth
  • Rabbit plasma
  • Simmons’ citrate agar slant
  • Spirit blue agar plate
  • Starch agar
  • Triple sugar iron agar slant
  • Tryptone broth

Reagents, Chemicals, Stains

  • Ammonium Sulfate (saturated)
  • Barritt’s A reagent
  • Barritt’s B reagent
  • Crystal Violet
  • Ferric chloride (10%) reagent
  • Gram’s Decolorizer
  • Gram’s Iodine
  • HCl 0.1N reagent
  • Hydrogen peroxide
  • Iodine reagent
  • Kovac’s reagent
  • Methyl red reagent
  • Nitrate A reagent
  • Nitrate B reagent
  • Oxidase reagent
  • Rabbit Plasma
  • Safranine

Gelatin hydrolysis test

About this test

What is the purpose of the test?  

The purpose is to see if the microbe can use the protein gelatin as a source of carbon and energy for growth. Use of gelatin is accomplished by the enzyme gelatinase .

How is gelatinase activity determined?  

A medium containing gelatin is used. The presence of gelatinase is detected by the addition of saturated ammonium sulfate after a streak plate has been allowed to grow overnight.  The ammonium sulfate forms a cloudy precipitate in the medium when it reacts with the gelatin.  Gelatinase activity is visible where clear zones in the cloudy precipitate are found, indicating the gelatin has been hydrolyzed.

What medium is used?  

An example for Staphylococci might be Stone Gelatin Agar (also known as Staphylococcus Medium 110).  But 3% gelatin can be added to other media to allow detection in other microbes. 

How is the test performed?  

An inoculum from a pure culture is streaked for isolation on an agar plate containing 3% gelatin.  The streak plate is incubated at 35-37 C for 24 hours, at which time saturated ammonium sulfate is added to enable interpretation of results.

What reagents are added?  

Ammonium Sulfate (saturated).

Performing this test in the VUMIE Online lab

To view the gelatinase  test in VUMIE OnlineĀ© Online Lab, complete the following steps:

1. Open the Tests link above the Virtual lab and select the Gelatinase Video.

2. Press the Play button to watch the video showing the test being performed.

3. Observe the results. If the bacterium possesses gelatinase, clear halos will surround the colonies of the microbe.  A negative culture will not have clear halos.

4. Record the test result observed.


VUMIE is the flight simulator of microbiology labs.

Learn more about VUMIE
Updated on June 6, 2022
Acid from glucose

Powered by BetterDocs